A Review on Induced Mutagenesis of Stevia rebaudiana Bertoni

Miao Si Chiew, Kok Song Lai, Sobri Hussein, Janna Ong Abdullah


Stevia rebaudiana Bertoni in the Asteraceae family is commercially valuable and cultivated throughout the world due to the great demand for its steviol glycosides (SGs) contents particularly rebaudioside A. Previous studies confirmed that maximal content of SGs in stevia was achieved at or just before flowering, and delayed flowering with long days provide longer duration for steviol glycosides accumulation. However, there is no suitable stevia variety to be cultivated in Malaysia due to her short day length. Mutation induction, including gamma irradiation, had been shown to be useful for generating genetic variations as well as developing new plant varieties from which desired mutants were successfully selected. The use of mutagens, both physical and chemical, has helped in creating mutants that expressed the selected desirable traits. This paper presents some selected essential data available in extant scientific studies on stevia with the focus on application of gamma irradiation on stevia. Both established achievements and recent publications of gamma radiation on stevia were reviewed. Emphasis is on the exceptional potential of stevia through induced mutation approach especially by using gamma rays.

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Ali, A. A., Aboshosha, A. A., Kassem, M. K., EL-Dabaawy, E. I., & EL-Banna, A. N. (2015). Salinity tolerance and stevioside improvement of in vitro selected stevia (Stevia rebaudiana) mutants.

International Journal of Current Research in Bioscience and Plant Biology, 2,11-20.

Amano, E. (2006). Use of induced mutants in rice breeding in Japan. Plant Mutation Reports, 1, 21-24.

American Cancer Society. Retrieved from http://www.cancer.org/cancer/cancercauses/ othercarcinogens/athome/aspartame (Accessed on 10 August 2016).

Aney, A. (2013). Effect of gamma irradiation on yield attributing characters in two varieties of pea (Pisum sativum L.). International Journal of Life Science, 1, 241-247.

Armizatul, S. A. H., Azhar, K., & Nazrul, R. (2009). Influence of lengthening day on stevia vegetative growth. Transaction of the Malaysian Society of Plant Physiology, 18, 17-18.

Awad, A. S. A. (2009). Cytogenetic studies on Stevia rebaudiana produced by tissue culture and affected by gamma rays and drought. (Unpublished master’s dissertation). University College for Women (Arts, Science and Education), Ain-Shams University, Cairo, Egypt. Retrieved from https://inis.iaea.org/search/search.aspx?orig_q=RN:41079109

Benford, D. J., DiNovi, M., & Schlatter, J. (2006). Safety evaluation of certain food additives: steviol glycosides. WHO Food Additives Series, 5, 117-144.

Bondarev, N. I., Sukhanova, M. A., Reshetnyak, O. V., & Nosov, A. M. (2003). Steviol glycoside content in different organs of Stevia rebaudiana and its dynamics during ontogeny. Biologia Plantarum, 47, 261-264.

Brower III, R. J., Carlson, T. L., Dang, B., Gonzalez, M. D., Kennedy, M. M., & Knutson, N. E. (2014). Stevia plants with an increased rebaudioside D content. U. S. Patent Application No. 14/774, 440.

Carakostas, M. C., Curry, L. L., Boileau, A. C., & Brusick, D. J. (2008). Overview: The history, technical function and safety of rebaudioside A, a naturally occurring steviol glycoside, for use in food and beverages. Food and Chemical Toxicology, 46, 1-10.

Ceballos, H., Sánchez, T., Denyer, K., Tofiño, A. P., Rosero, E. A., Dufour, D., Smith, A., Morante, N., Pérez, J.C., & Fahy, B. (2008). Induction and identification of a small-granule, high-amylose mutant in cassava (Manihot esculenta Crantz). Journal of Agricultural and Food Chemistry, 56, 7215-7222.

Chatsudthipong, V., & Muanprasat, C. (2009). Stevioside and related compounds: Therapeutic benefits beyond sweetness. Pharmacology and Therapeutics, 121, 41-54.

Chen, X., Liu, X., Wu, D., & Shu, Q. Y. (2006). Recent progress of rice mutation breeding and germplasm enhancement in China. Plant Mutation Reports, 1, 4-6.

Debnath, M. (2008). Clonal propagation and antimicrobial activity of an endemic medicinal plant

Stevia rebaudiana. Journal of Medicinal Plants Research, 2, 45-51.

DuBois, G. E. (2000). Sweeteners: non-nutritive. In: Francis, F. J. Wiley Encyclopedia of Food Science and Technology, Vol. 4, 2nd ed., John Wiley & Sons, New York, 2245-2265.

DuBois, G. E., & Prakash, I. (2012). Non-caloric sweeteners, sweetness modulators, and sweetener enhancers. Annual Review of Food Science and Technology, 3, 353-380.

Forster, B. P., Shu, Q. Y., & Nakagawa, H. (2012). Plant mutagenesis in crop improvement: Basic terms and applications. Plant Mutation Breeding and Biotechnology, 9-20.

Goyal, S. K., & Goyal, R. K. (2010). Stevia (Stevia rebaudiana) a bio-sweetener: A review. International Journal of Food Sciences and Nutrition, 61, 1-10.

Gupta, E., Purwar, S., Sundaram, S., & Rai, G. K. (2013). Nutritional and therapeutic values of Stevia rebaudiana: A review. Journal of Medicinal Plants Research, 7, 3343-3353.

Hasbullah, N. A., Taha, R. M., Saleh, A., & Mohamed, N. (2012). Physiological responses of callus from Gerbera jamesonii Bolus ex. Hook f. to gamma irradiation. Brazilian Archives of Biology and Technology, 55, 411-416.

Hsieh, M. H., Chan, P., Sue, Y. M., Liu, J. C., Liang, T. H., Huang, T. Y., Tomlinson, B., Chow, M. S., Kao, P. F., & Chen, Y. J. (2003). Efficacy and tolerability of oral stevioside in patients with mild essential hypertension: A two-year, randomized, placebo-controlled study. Clinical Therapeutics, 25, 2797-2808.

Hussein, S., Pick, A., Ling, K., Yang, C., Ling, S., Abdul, O., & Harun, R. (2012). Morphological and biochemical responses of Eurycoma longifolia callus to gamma irradiation, Bioremediation, Biodiversity and Bioavailability, 6, 70-74.

International Diabetes Federation. Retrieved from http://www.idf.org/membership/wp/Malaysia (Accessed on 27 February 2016).

Jain, S. M. (2005). Major mutation-assisted plant breeding programs supported by FAO/IAEA. Plant Cell, Tissue and Organ Culture, 82, 113-123.

Jain, S. M. (2010). Mutagenesis in crop improvement under the climate change. Romanian Biotechnological Letters, 15, 88-106.

JakinovichJr, W., Moon, C., Choi, Y. H., & Kinghorn, A. D. (1990). Evaluation of plant extracts for sweetness using the Mongolian gerbil. Journal of Natural Products, 53, 190-195.

Janick, J. (2015). Plant breeding reviews (Vol. 39). John Wiley & Sons. Retrieved from https://books.google.com.my

Jayaraman, S., Manoharan, M. S., & Illanchezian, S. (2008). In-vitro antimicrobial and antitumor activities of Stevia rebaudiana (Asteraceae) leaf extracts. Tropical Journal of Pharmaceutical Research, 7, 1143-1149.

Kangarasu, S., Ganeshram, S., & Joel, A. J. (2014). Determination of lethal dose for gamma rays and ethyl methane sulphonate induced mutagenesis in cassava (Manihot esculenta

Crantz.). International Journal of Scientific Research, 3, 3-6.

Kedik, S. A., Yartsev, E. I., & Stanishevskaya, I. E. (2009). Antiviral activity of dried extract of Stevia. Pharmaceutical Chemistry Journal, 43, 198–199.

Khalil, S. A., Ahmad, N., & Zamir, R. (2015). Gamma radiation induced variation in growth characteristics and production of bioactive compounds during callogenesis in Stevia rebaudiana

(Bert.). New Negatives in Plant Science, 1, 1-5.

Khalil, S. A., Zamir, R., & Ahmad, N. (2014). Effect of different propagation techniques and gamma irradiation on major steviol glycoside’s content in Stevia rebaudiana. Journal of Animal and Plant Science, 24, 1743-1751.

Khan, S. J., Khan, H. U., Khan, R. D., Iqbal, M. M., & Zafar, Y. (2000). Development of sugarcane mutants through in vitro mutagenesis. Pakistan Journal of Biological Sciences, 3, 1123-1125.

Khan, S. A., urRahman, L., Verma, R., & Shanker, K. (2016). Physical and chemical mutagenesis in Stevia rebaudiana: Variant generation with higher UGT expression and glycosidic profile but with low photosynthetic capabilities. Acta Physiologiae Plantarum, 38, 1-12.

Kim, J. Y., Seo, J., & Cho, K. H. (2011). Aspartame-fed zebrafish exhibit acute deaths with swimming defects and saccharin-fed zebrafish have elevation of cholesteryl ester transfer protein activity in hypercholesterolemia. Food and Chemical Toxicology, 49, 2899-2905.

Kodym, A., & Afza, R. (2003). Physical and chemical mutagenesis. Plant Functional Genomics, 236, 189-203.

Kovacs, E., & Keresztes, A. (2002). Effect of gamma and UV-B/C radiation on plant cells. Micron, 33, 199-210.

Lai, Y. P., Huang, J., Wang, L. F., Li, J., & Wu, Z. R. (2004). A new approach to random mutagenesis in vitro. Biotechnology and Bioengineering, 86, 622-627.

Lemus-Mondaca, R., Vega-Gálvez, A., Zura-Bravo, L., & Ah-Hen, K. (2012). Stevia rebaudiana Bertoni, source of a high-potency natural sweetener: A comprehensive review on the biochemical, nutritional and functional aspects. Food Chemistry, 132, 1121-1132.

Lester, T. (1999). Stevia rebaudiana sweet leaf. The Australian New Crops Newsletter, 11, 1. Maamoun, M. K. M., El-Mahrouk, M. E., Dewir, Y. H., & Omran, S. A. (2014). Effect of radiation and chemical mutagens on seeds germination of black cumin (Nigella sativa L). Journal of Agricultural Technology, 10, 1183-1199.

Mba, C. (2013). Induced mutations unleash the potentials of plant genetic resources for food and agriculture. Agronomy, 3, 200-231.

Mba, C., Afza, R., Bado, S., & Jain, S. M. (2010). Induced mutagenesis in plants using physical and chemical agents. Plant Cell Culture: Essential Methods, 20, 111-130.

Mutant Varieties Database of the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture. Available online: http://mvgs.iaea.org/AboutMutantVarities.aspx (Accessed on 27 February 2016).

Novak, F. J., & Brunner, H. (1992). Plant breeding: Induced mutation technology for crop improvement. IAEA Bulletin, 4, 25-33.

Nurhidayah, S., Norazlina, N., & Rusli, I. (2014). Effect of acute gamma irradiation on in vitro growth of Stevia rebaudiana Bertoni. Innovative Plant Productivity and Quality, 22, 214-217.

Pande, S., & Khetmalas, M. (2011). Effect of gamma irradiations on seed germination and seedling survival of Stevia rebaudiana Bert. South Asian Journal of Experimental Biology, 1, 255-259.

Patrick, L. (2014) Malaysia is fattest country in South-East Asia. In: The Star Online. Retrieved from http://www.thestar.com.my/News/Nation/2014/01/16/Scoring-badly-on-dietary-habit-Malaysia-is-fattest-country-in-S E-Asia/ (Accessed on 27 February 2016).

Philippe, R. N., De Mey, M., Anderson, J., & Ajikumar, P. K. (2014). Biotechnological production of natural zero-calorie sweeteners. Current Opinion in Biotechnology, 26, 155-161.

Predieri, S. (2001). Mutation induction and tissue culture in improving fruits. Plant Cell, Tissue and Organ Culture, 64, 185-210.

Rajarajan, D., Saraswathi, R., & Sassikumar, D. (2016). Determination of lethal dose and effect of gamma ray on germination percentage and seedling parameters in ADT (R) 47 Rice. International Journal of Advanced Biological Research, 6, 328–332.

Ramesh, K., Singh, V., & Megeji, N. W. (2006).Cultivation of stevia [Stevia rebaudiana (Bert.) Bertoni]: A comprehensive review. Advances in Agronomy, 89, 137-177.

Rameshsing, C. N., Hegde, S. N., & Vasundhara, M. (2015). Enhancement of steviol glycosides in stevia (Stevia rebaudiana Bertoni) through induction of polyploidy. Current Trends in Biotechnology and Pharmacy, 9, 141-146.

Roslim, D. I., & Fiatin, I. (2015). Lethal dose 50 (LD 50) of mungbean (Vigna radiata L. Wilczek) cultivar Kampar. Sabrao Journal of Breeding and Genetics, 47, 510-516.

Sattigeri, B. M. (2012). Stevia (Madhu Patra) – A natural low calorie sweetener with more promising benefits. International Journal of Phytothearpy Research, 2, 20-25.

ŠicŽlabur, J., Voća, S., Dobričević, N., Ježek, D., Bosiljkov, T., & Brnčić, M. (2013). Stevia rebaudiana Bertoni-A review of nutritional and biochemical properties of natural sweetener. Agriculturae Conspectus Scientificus (ACS), 78, 25-30.

Silva, P. A., Oliveira, D. F., Prado, N. R. T. D., Carvalho, D. A. D., & Carvalho, G. A. D. (2008). Evaluation of the antifungal activity by plant extracts against Colletotrichum gloeosporioides Penz. Ciência e Agrotecnologia, 32, 420-428.

Snehal, P. & Madhukar, K. (2011).Biological effect of gamma irradiations on in vitro culture of

Stevia rebaudiana. Indian Journal of Applied Research, 1, 11-12.

Soejarto, D. D., Compadre, C. M., Medon, P. J., Kamath, S. K., & Kinghorn, A. D. (1983). Potential sweetening agents of plant origin. II. Field search for sweet-tasting stevia species. Economic Botany, 37, 71-79.

Songsri, P., Suriharn, B., Sanitchon, J., Srisawangwong, S., & Kesmala, T. (2011). Effects of gamma radiation on germination and growth characteristics of physic nut (Jatropha curcas L.).

International Journal of Biological Sciences, 11, 268-274.

Summon, M. H., Mostofa, M., Jahan, M. S., Kayesh, M. E. H., & Haque, M. A. (2008). Comparative efficacy of powdered form of Stevia (Stevia rebaudiana Bertoni) leaves and glimepiride in induced diabetic rats. Bangladesh Journal of Veterinary Medicines, 6, 211-215.

Takahashi, K., Matsuda, M., Ohashi, K., Taniguchi, K., Nakagomi, O., Abe, Y., Mori, S., Sato, N., Okutani, K., & Shigeta, S. (2001). Analysis of antirotavirus activity of extract from Stevia rebaudiana. Antiviral Research, 49, 15–24.

Toker, C., Yadav, S. S., & Solanki, I. S. (2007). Mutation breeding. In: Yadav, S. S., McNeil, D., & Stevenson, P. C. (eds) Lentil: An Ancient Crop for Modern Times. Springer Dordrecht, The Netherlands, pp. 209-224.

Tomlekova, N. B. (2010). Induced mutagenesis for crop improvement in Bulgaria. Plant Mutation Reports, 2, 4-27.

Ulukapi, K., & Nasircilar, A. G. (2015). Developments of gamma ray application on mutation breeding studies in recent years. In: International Conference on Advances in Agricultural, Biological & Environmental Sciences (AABES-2015). London, United Kingdom, 31-34.

Wi, S. G., Chung, B. Y., Kim, J. S., Kim, J. H., Baek, M. H., Lee, J. W., & Kim, Y. S. (2007). Effects of gamma irradiation on morphological changes and biological responses in plants. Micron, 38, 553-564.

Wiebe, N., Padwal, R., Field, C., Marks, S., Jacobs, R., & Tonelli, M. (2011). A systematic review on the effect of sweeteners on glycemic response and clinically relevant outcomes. BMC Medicine, 9, 1.

Yadav, A. K., Singh, S., Dhyani, D., & Ahuja, P. S. (2011). A review on the improvement of stevia [Stevia rebaudiana (Bertoni)]. Canadian Journal of Plant Science, 91, 1-27.


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